2 Answers. Supplier: MP Biomedicals. 2. ,IC10113005EA,IC10113025EA. Meloun et al. Bromothymol Blue sodium salt powder Synonym: 3′,3′′-dibromothymolsulfonephthalein sodium salt CAS Number 34722-90-2. Answer Save. Computational methods, such as SQUAD (84) 16 and SPECFIT/32, and some new methods Acid - Base indicators are either weak acids or weak bases so they exist as molecules in equilibrium with their ions. pH = pKa - log ( [HIn] / [In-] ) … (2) Beilstein/REAXYS Number 6887926 . Given the equation: y= 48048x- 0.0105, find the molar absorptivity and use this value in your experiment. The indicator (HIn) is a monoprotic acid and we can represent its dissociation as follows: HIn → H+ + In- … (1) The equilibrium expression for such dissociation can be written as. The pKa′ value was determined using a method similar to that of Reilley and Swayer. Indicator: pH Range: pKa: Acid Form: Base Form: Methy Violet: 0.0 - 1.6: 0.8: Yellow: Blue: Thymol Blue: 1.2 - 2.8: 1.6: Red: Yellow: Methyl Yellow: 29. Place three small drops of bromothymol blue solution into each of the 5 cuvettes. Molecular Weight 646.36 . Description: Bromothymol Blue (BTB) is a pH indicator (pKa approx. PubChem Substance ID 24892083. Other indicators work in different pH ranges. Solutions of different concentration of the indicator were used. [10] [11] describe multiwavelength analysis and nonlinear least squares regres-sion methods. The pKa can be determined from the spectrophotometric data using different methods. Into each of the cuvettes place 3 mL of the solutions of the various pH values (hydrochloric acid, the buffer solutions with pH values 6.0, 7.0 and 8.0, and sodium hydroxide solution). Relevance. Molar absorptivity ___ (You will be using this value in your experiment.) Spectrophotometric determination of the pKa of Bromothymol Blue. Explain why the acid - base indicator bromothymol blue turns yellow when added to dilute HCl but blue when added to dilute NaOH. EC Number 252-169-7. As a pH indicator,bromothymol blue, for example, would be useful between from about pH6.0 to pH 7.6. It shows that pH=pKa where [ln-]= [Hln] or hwere ( [ln-]/ [Hln])=1 or where log ( [ln-]/ [Hln])=0. NACRES NA.47 7.1) that is used as a biological slide vital stain to define cell walls or nuclei under the mic... SDS. Anonymous. MDL number MFCD00077263. Assume a cuvet with a 14.45 mm diameter was used. Using bromothymol blue you wouldn't be able to distinquish between anacid with pH 4 from an acid with pH 5. They would both appearyellow. Ensure that the solutions are mixed well. In this experiment, spectrophotometry is employed to measure the pKa of bromothymol blue, an acid-base indicator. There is a linear relationship between log ( [ln-]/ [Hln]) and pH. Bromothymol blue is an acidic pH chromoionophore where acid to base change corresponds to its neutral to anionic form, respectively. For this reason it can be electrostatically immobilized on the surface on anion-exchanger resin like Amberlite IRA 401. Empirical Formula (Hill Notation) C 27 H 27 Br 2 NaO 5 S . Chloroform showed the highest affinity for bromothymol blue, followed by methylene chloride, ethylene dichloride, benzene, toluene, and carbon tetrachloride. 1 decade ago. Bromothymol blue, Powder ACS. Favorite Answer. Determination of the pK_a of Bromothymol Blue Use the following graph to determine the molar absorptivity of Bromothymol blue (BTB)? , and carbon tetrachloride a pH indicator, bromothymol blue, followed by methylene chloride, ethylene,! An acid-base indicator Br 2 NaO 5 S 4 from an acid with 5. 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